The state, with just 7% of the US population, surpassed the previous daily record held by California.
Florida, which began lifting coronavirus restrictions in May, has proved vulnerable due to tourism and an elderly population.
Its figures eclipse the worst daily rates seen in New York in April.
Florida also registered an additional 45 deaths.
The state would rank fourth in the world for new cases if it were a country, according to a Reuters analysis. More than 40 hospitals in Florida say their intensive care facilities are at full capacity.
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